PCR in neuroscience [electronic resource]

• 其他作者：
  • Sarkar, Gobinda.
  • ScienceDirect (Online service)
• 其他題名：
  • Polymerase Chain Reaction in neuroscience.
• 出版： San Diego : Academic Press
• 叢書名： Methods in neurosciencesv. 26
• 主題： Polymerase chain reaction , Neurosciences , Polymerase Chain Reaction--methods--Laboratory Manuals. , Nervous System--chemistry--Laboratory Manuals. , Neurosciences--methods--Laboratory Manuals. , Bioquimica. , DNA , Electronic books.
• ISBN： 9780121852962 (paper) 、 0121852962 (paper)
• FIND@SFXID: CGU
• 資料類型: 電子書
• 內容註: Includes bibliographical references and index. Direct PCR on Clinical Specimens: -- M. Panaccio, M. French, and A. Lew, Formamide Low-Temperature PCR: Applications for Direct PCR from Clinical Material. -- B.-Y. Nordveg, H.M.F. Riise, G. Husby, I. Nilsen, and M.R.El-Gewely, Direct Use of Blood in PCR. -- Application of Reverse Transcription-Mediated PCR: -- T.K. Chatterjee, G. Sarkar, M.E. Bolander, and R.A. Fisher, Use of PCR for Isolation of Neuropeptide Receptor Genes. -- N.D. Borson, W.L. Salo, and L.R. Drewes, Lock-Docking Rapid Amplification of cDNA Ends PCR: Strategies and Applications. -- B.L. Ziegler, C.P. Lamping, S.J. Thoma, C.A. Thomas, and T.M. Fliedner, Single-Cell cDNA-PCR. -- Gene Synthesis by PCR: -- A. Darveau, A. Pelletier, and J. Perreault, PCR-Mediated Synthesis of Chimeric Molecules. -- K. Jayaraman, PCR-Mediated Gene Synthesis. -- Quantitative PCR: -- N.W. Soong and N. Arnheim, Quantitative PCR: Analysis of Rare Mitochondrial DNA Mutations in Central Nervous System Tissues. -- M. Becker-Andro, PCR-Aided Transcript Titration Assay: Competitive PCR for Evaluation of Absolute Levels of Rare mRNA Species. -- S. Sur, G.J. Gleich, M.E. Bolander, and G. Sarkar, Comparative Evaluation of Quantitative PCR Methods. -- Application of PCR in Mutation Detection: -- N. Bardeesy and J. Pelletier, Mutational Detection by Single-Strand Conformational Polymorphism. -- A. Sanyal and G.S. Getz, Detection of Mutation in Yeast hsp60 Gene by PCR. -- D. Glavac[breve] and M. Dean, Comparison of the Sensitivity of Single-Strand Conformational Polymorphism and Heteroduplex Methods. -- T.M. Cheng, V. Ganju, S.R. Ritland, G. Sarkar, and R.B. Jenkins, Analysis of p53 Mutations in Human Gliomas by RNA Single-Strand Conformational Polymorphism. -- Generation of Probes by PCR: -- H. Yang and P.W. Melera, PCR and Generation of Antisense RNA Probes for Use in RNase Protection Assays. -- S. Leonard, In Vitro Transcription of cRNA from PCR-Generated DNA Fragments. -- PCR in the Context of Cloning and Constructing Libraries
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• 摘要註: The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Key Features * Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) * Complete retrieval of novel expressed genes by PCR without screening a library * Quantitation by PCR * Mutagenesis by PCR * PCR in AIDS research * Simple and effective protocols for PCR on archival specimens.
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