Microbiology of atypical environments [electronic resource]

• 其他作者：
  • Gurtler, Volker, editor.
  • Trevors, Jack T., 1953- editor.
• 其他題名：
  • Methods in microbiology ;
• 出版： London, United Kingdom : Academic Press, an imprint of Elsevier
• 版本：First edition.
• 叢書名： Methods in microbiology ;Volume 45
• 主題： Microbiology. , Microorganisms. , Microbiology. , Electronic books.
• ISBN： 9780128146057 (electronic bk.) 、 0128146052 (electronic bk.)
• FIND@SFXID: CGU
• 資料類型: 電子書
• 內容註: Includes bibliographical references. Front Cover; Microbiology of Atypical Environments; Copyright; Contents; Contributors; Cover Picture; Preface; Section 1: Methods for Studying Microorganisms in Space; Chapter 1: Microbiology of the Built Environment in Spacecraft Used for Human Flight; 1. Introduction; 2. Microbiological Monitoring in Human-Occupied Spacecraft; 2.1. Microorganisms in Human-Occupied Spacecraft; 3. Ground-Based Tests (Simulating Microgravity in the Laboratory); 4. Microorganisms Associated With Water Recovery; 5. Relevance of the Spacecraft Microbiome to Humans during Space Exploration 6. Microbial Influences on Spacecraft Integrity and Function7. Biofilms and Their Impact on Spaceflight; 8. Long-Term Studies; Conclusions; Future Directions; Acknowledgements; References; Chapter 2: The Study of Microbial Survival in Extraterrestrial Environments Using Low Earth Orbit and Ground-Based Experimen; 1. Introduction; 2. Microbiological Facilities in Space: Past and Present; 2.1. The Long Duration Exposure Facility; 2.2. EUropean REtrievable Carrier; 2.3. BIOPAN; 2.4. EXPOSE Facility; 2.5. Organism/Organic Exposure to Orbital Stresses Nanosatellite 3. Ground-based Simulation Facilities3.1. Simulated Mars Conditions; 3.2. Simulated Space Conditions; 3.3. Simulating Lithopanspermia; 3.3.1. Light gas gun; 3.3.2. Shock recovery experiments; 3.3.3. ESAs STONE experiment; 4. Postexposure Analyses; 4.1. Microscopic Analysis; 4.2. Metabolic Analysis; 4.3. Molecular Analyses; 5. Life Detection; 5.1. Spectroscopic Methods; 5.1.1. Infrared spectroscopy; 5.1.2. UV-visible spectroscopy; 5.1.3. Raman spectrometry; 5.2. Gas Chromatography-Mass Spectrometry; Conclusion; References; Further Reading Chapter 3: Persistence of Fungi in Atypical, Closed Environments: Cultivation to Omics1. Introduction; 2. International Space Station and Other Confined Facilities as Microbial Habitats; 2.1. Microbial Sample Collection and Initial Processing; 3. Progression From Culture- to Genome-Based Approach in Microbial Characterization of Confined Spaces; 3.1. Culture-Based Microbial Analysis; 3.2. DNA-Based Microbial Analyses; 3.2.1. Pyrosequencing- and Illumina-derived fungal diversity; 3.2.2. Metagenomics-derived fungal diversity and functional characterization; Conclusions and Perspectives AcknowledgementsReferences; Section 2: Molecular Methods; Chapter 4: Molecular Methods for Studying Microorganisms From Atypical Environments; 1. Introduction; 2. Current Status of Microbial Diversity at Atypical Environments; 3. Application of Molecular Tools for Studying Atypical Microbial Diversity; 3.1. Whole Community Analysis; 3.1.1. DNA-DNA hybridization (DDH); 3.1.2. Analysis of G+C content; 3.1.3. Complete microbial genome sequencing; 3.1.4. Use of metagenomics; 3.2. Partial Community Analysis; 3.2.1. Clone library method; 3.2.2. Genetic fingerprinting techniques
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